PEPTIDES4ALL PNC-27 – Targeted Anti-Cancer Peptide for Advanced Research Applications

PEPTIDES4ALL PNC-27 is a synthetic peptide engineered to selectively target and destroy cancer cells while sparing healthy, untransformed tissue. It incorporates a p53-derived sequence segment (amino acids 12–26) fused to a membrane-penetrating domain, allowing it to bind to HDM-2 proteins abnormally expressed on the surface membranes of malignant cells. Upon binding, PNC-27 forms transmembrane pores that compromise cellular integrity, leading to rapid necrosis of tumor cells without affecting normal cells.

This unique mechanism of action has drawn significant attention in oncology research as a novel model for selective tumor cell killing independent of conventional chemotherapeutic pathways. Unlike most cytotoxic agents, PNC-27 operates independently of p53 functionality, enabling it to eliminate tumor cells lacking active or wild-type p53, a common feature in many aggressive and treatment-resistant cancers.

Both in vitro and in vivo studies have demonstrated its broad-spectrum cytotoxicity across multiple tumor types—including colon, pancreatic, lung, breast, prostate, ovarian, leukemia, and osteosarcoma models—confirming its reproducible selectivity and potency (PubMed;  PMC). In hematologic research, PNC-27 has shown pronounced activity in leukemia cell lines, suggesting therapeutic potential through HDM-2 membrane targeting and pore-forming cytolysis (Anticancer Research Journal).

With its precise molecular design and reproducible biological effects, PEPTIDES4ALL PNC-27 serves as a powerful research tool for advancing studies in tumor biology, molecular pharmacology, and experimental oncology, helping to illuminate new mechanisms of selective cell death and potential targets for next-generation anti-cancer therapeutics.

Scientific Evidence & Research Findings

• Selective Tumor Cell Killing – PNC-27 binds to HDM-2 expressed on cancer cell membranes, co-localizes there, and induces pore formation to kill tumor cells — without harming normal (untransformed) cells. https://pmc.ncbi.nlm.nih.gov/articles/PMC2836618/

• Broad Cancer Spectrum – Demonstrated cytotoxicity in cancers such as colon, prostate, lung, pancreatic, breast, leukemia, ovarian, and osteosarcoma models. https://pubmed.ncbi.nlm.nih.gov/25117093/

• Activity in p53-Deficient Cells – Because its mechanism is independent of p53, PNC-27 can kill tumor cells lacking functional p53, such as certain leukemias. https://pubmed.ncbi.nlm.nih.gov/25117093/

• Anti-Leukemia Action – PNC-27 targeting of membrane HDM-2 showed significant activity in leukemia cell lines in lab models. https://ar.iiarjournals.org/content/40/9/4857

Key Research Benefits & Applications

• Selective Cancer Cell Cytotoxicity: Targets HDM-2 proteins abnormally expressed on tumor cell membranes, forming transmembrane pores that induce rapid necrotic death while leaving normal cells unharmed.

• p53-Independent Mechanism: Demonstrates tumor selectivity regardless of p53 status, enabling effective cytolytic action against mutant or p53-deficient cancers resistant to traditional therapies.

• Broad Antitumor Spectrum: Exhibits reproducible cytotoxic activity across solid and hematologic malignancies, including pancreatic, colon, lung, breast, ovarian, prostate, leukemia, and osteosarcoma models.

• Membrane-Targeted Therapeutic Model: Provides a novel framework for HDM-2–mediated targeting, helping elucidate membrane-bound protein roles in selective cytolysis and cancer cell vulnerability.

• Non-Toxic to Healthy Tissue: Its selective pore-forming mechanism spares normal, untransformed cells—offering a research model for next-generation precision cytotoxic peptides.

• Applications in Oncology & Molecular Pharmacology: Supports investigation into membrane protein targeting, tumor cell recognition, and peptide-induced necrosis, advancing development of target-specific cancer therapeutics.

Presentation & Handling

• Form: Supplied as lyophilized peptide in sterile vial.
• Reconstitution: Use bacteriostatic water or 2% procaine; swirl gently (do not shake vigorously).
• Storage: Store at –20 °C, protected from light and moisture. After reconstitution, use according to research protocols and handle under sterile conditions.

Intended Use

For research purposes only. Must be handled in accordance with institutional protocols and ethical guidelines.